Results of Frequency Analysis Distribution of Polymorphism Rs1800895 592c>A In Il10 Gene among Patients with Chronic Polypoid Rhinosinusitis

Abstract

Introduction. Chronic polyposis rhinosinusitis is a disease characterized by chronic inflammation of the nasal mucosa and sinuses. In the tissues of polyps and intranasal secretions, an increase in the concentration of various inflammatory mediators, in particular interleukins, is observed due to an increase in their de novo synthesis by effector cells.

The research objective to study the distribution of genotypic forms of the IL10 gene locus and evaluate their role in the development and clinical course of chronic polypoid rhinosinusitis.

Material and methods. In accordance with the purpose of the study and to fulfill the assigned tasks, clinical studies were carried out in 140 patients with CPRS and with chronic rhinosinusitis, who were examined and treated at the ENT department of the multidisciplinary clinic of the Tashkent Medical Academy in 2017-2019. The examined patients met the following criteria: the presence of polyposis tissue in the nasal cavity that obstructs the common nasal passage completely or by at least 50%; complaints of prolonged difficulty in nasal breathing; according to the patient, the disease significantly reduces the quality of his life; absence of acute inflammatory pathology; written informed consent for surgical treatment and morphological examination of the surgical material.

Results and discussion. The revealed reliably high frequency of this genotype in group 1 of patients with CPRS, compared with group 2 of CPRS, also confirms our assumption about the protective role of the genotypic variant of the A / A polymorphism rs1800895 592 C> A in the IL10 gene, in relation to the development of CPRS in patients (6.45 % versus 5.0%, respectively, at χ2 = 0.02; Р = 0.45; RR = 1.17; OR = 1.18; 95% CI: 8.557-8.942). This fact is probably due to the fact that in carriers of the A / A genotype of the IL10 gene, the anti-inflammatory activity of epithelial matrix metalloproteinases is significantly increased, compared with patients carrying other genotypic variants.

Conclusion. The results obtained showed that, with this pathology of the genotypic variant of the A / A polymorphism rs1800895 592 C> A in the IL10 gene, there is a risk of developing CPRS in patients (6.45% versus 5.0%, respectively). This fact is probably due to the fact that in carriers of the A / A genotype of the IL10 gene, the anti-inflammatory activity of epithelial matrix metalloproteinases is significantly increased, compared with patients carrying other genotypic variants.

KEYWORDS: polyposis rhinosinusitis, polymorphism, genotype, nasal mucosa, cytokine